Application

Research Sub CategoryCardiovascular DiseaseMetabolic Disorders

Research CategoryMetabolism

Biochem/physiol Actions

Protein Target: haspin

Target Sub-Family: Misc

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells.

Product Source: Recombinant enzyme expressed in Sf21 cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

10 µg of enzyme in 6.3 µL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Purified using Ni²⁺/NTA agarose. Purity 83% by SDS-PAGE and Coomassie blue staining.

Quality

Routinely evaluated by phosphorylation of 500 µM RARTLSFAEPG.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3µg of Haspin.

Storage and Stability

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Unit Definition

Specific Activity: where one unit of Haspin activity is defined as 1 nmol phosphate incorporated into 500 µM RARTLSFAEPG per minute at 30°C with a final ATP concentration of 100 µM.